通过比较D-101、AB-8、X-5、DA-201 4种大孔吸附树脂对景天三七总黄酮的静态吸附和解吸附性能,筛选出最适合的树脂及最佳静态吸附-解吸附条件。对纯化后的总黄酮进行初步鉴定,并考察了纯化前后的体外抗氧化效果。结果表明,DA-201型大孔吸附树脂的吸附和解吸附效果最好,其最佳静态吸附分离工艺参数为:吸附时间为1 h,样液质量浓度为0.7 mg/mL,上样液pH为3.0,解吸附乙醇浓度为90%。在该条件下静态吸附率为81.05%,解吸附率为86.05%。纯化后,总黄酮的纯度由19.38%提高到43.78%,其羟自由基、DPPH自由基清除率的IC50分别由69.47、44.02 μg/mL下降到43.25、30.16 μg/mL。另外初步判断纯化后的景天三七总黄酮可能含有水飞蓟素类、金丝桃苷类、山奈酚类、槲皮素类化合物。
By comparing 4 kinds of macroporous adsorption resins D-101, AB-8, X-5 and DA-201, the static adsorption and adsorption properties of total flavonoids from Sedum aizoon, the most suitable resin and the best static adsorption desorption conditions were selected. The total flavonoids after purification were identified and the antioxidant effects were investigated before and after purification. The results of static adsorption test showed that the adsorption and adsorption of DA-201 macroporous adsorption resin were the best, the optimum static adsorption separation process parameters were as follows:The adsorption time was 1 h, the mass concentration of the sample solution was 0.7 mg/mL, the pH value of the sample solution was 3.0, and the desorption concentration of ethanol was 90%. Under this condition, the static adsorption rate was 81.05% and desorption rate was 86.05%. After purification, the purity of total flavonoids increased from 19.38% to 43.78%. The hydroxyl radical and DPPH free radical clearance rate of IC50 decreased from 69.47, 44.02 μg/mL to 43.25, 30.16 μg/mL respectively. In addition, it was preliminarily judged that the total flavones of the prepared cabbage might contain silymarin, hypericin, kaempferol and quercetin.