气流喷爆-复合酶解对豆粕中水溶性膳食纤维的提取优化

Optimization of the Modification Process of Water-soluble Dietary Fibers in Soybean Meal by Blasting Extrusion Processing-complex Enzyme Hydrolysis

摘要: 本研究旨在通过气流喷爆-复合酶解的处理方式高效回收豆粕中的水溶性膳食纤维。酶法制油豆粕先经气流喷爆处理,再经纤维素酶及α-淀粉酶复合酶解作用,并通过响应面法对其工艺参数进行优化。结果表明,气流喷爆-复合酶解处理后,在喷爆温度为220℃、喷爆时间30 s、纤维素酶:α-淀粉酶(g:g)为2:1、酶解时间2 h时,水溶性膳食纤维得率最佳,为26.03%±0.02%。扫描电镜观测处理后豆粕结构疏松,排列更加规整。

Abstract: The aim was to efficiently recover water-soluble dietary fiber in soybean meal by means of blasting extrusion processing-complex enzyme hydrolysis. Soybean meal by oil enzymatically produced was firstly sprayed and blasted,then hydrolyzed by cellulase and α-amylase,and its process parameters were optimized by response surface methodology. The results showed that the yield of water-soluble dietary fiber was the best,which was 26.03%±0.02% after the air blasting-complex enzymatic modification treatment of the explosion temperature at 220℃,the blasting time at 30 s,the cellulose:α-amylase(g:g)at 2:1 and the enzymatic time at 2 h. Scanning electron microscopy observation of modified soybean meal structure was loose and the arrangement was more regular.