Abstract: Objective:The aim of this study was to investigate the protective effect of resveratrol(RSV)on the rat INS-1 cell injury induced by advanced glycation end products(AGEs)and the underlying mechanisms of anti-inflammatory and anti-oxidative stress involved in it. Methods:In this experiment,the glycation serum(GS)which included various AGEs was used to mimic the diabetes settings and to establish a cell injured model. INS-1 cells were divided into control group,20% GS model group,20% GS+RSV groups with different concentrations. The cell proliferation rates was detected by MTT assay. The intracellular SOD activities,GSH contents,supernatant NO,intracellular ROS,MDA contents and supernatant TNF-α were measured by Kits. Results:Compared with control group,the model group cells proliferation rate were significantly decreased(p<0.01).The SOD activities and the levels of GSH in model group were significantly decreased(p<0.01). The levels of NO and ROS in model group were significantly increased(p<0.01). The levels of MDA in model group were significantly increased(p<0.05).The levels of TNF-α in model group were significantly increased(p<0.01). Compared with model group,the proliferation rates of INS-1 cells of 25 and 50 μmol/L RSV group were significantly increased(p<0.01),and the content of GSH and SOD activities were significantly increased(p<0.01),the contents of NO were significantly decreased(p<0.01). In addition,the levels of MDA were significantly reduced(p<0.05)in 25 μmol/L RSV group and the levels of ROS were significantly reduced(p<0.05)in 50 μmol/L RSV group. The contents of TNF-α were significantly decreased(p<0.01). Conclusion:RSV has a significant protective effect on the AGEs injured INS-1 cells,and the mechanism would be related to improve the anti-oxidative stress and anti-inflammatory abilities of INS-1 cells.