Abstract: In order to study the effect of liquid culture conditions on mycelial growth of Ramaria botrytis,eight factors included carbon source,nitrogen source,inorganic ion combination,growth factor,initial pH of culture medium,culture temperature,shaking speed and light were investigated by single factor and orthogonal experiment. The results showed that,the optimum culture conditions were as follows:At 23℃ and shaking speed of 220 r/min,initial pH6.5,flask volume 50 mL/250 mL erlenmeyer flask,inoculum density 10%(V/V),full darkness culture for 7 days,the optimum liquid culture medium for 1L was 25 g soluble starch,3.5 g yeast extract,4.4 g(1.2 g KH₂PO₄+1.6 g FeSO₄·7H₂O+1.6 g MgSO₄·7H₂O)inorganic ion combination and 0.01 g VB₁,under the above conditionsthe mycelial growth of R. botrytis was(26.8±0.29) g/L,the result laid the foundation for liquid fermentation of R. botrytis.