Abstract: In this paper,corn syrup(dry and wet samples)was taken as sample. The method of organic solvent extraction was used to simplify the sample processing and the recovery rates of aflatoxin B₁ from 4 organic solvents were compared to develop a high performance liquid chromatography(HPLC)method for the determination of AFB₁ in corn syrup. The results showed that,dichloromethane was the optimum extractant for sample detection,and the recovery rate of this method was 96.2%~99.3%,the relative standard deviation(RSD)was 0.68%~1.97%. The method was simple,cheap,accurate and suitable for the detection of AFB₁ in corn syrup.