细胞膜固相色谱法筛查奶粉中AGEs特异结合成分

Screen of AGEs Specific Binding Components in Milk Powder by Cell Membrane Immobilized Chromatography

摘要: 采用细胞膜固相色谱技术检测食品中的晚期糖基化终产物。以含晚期糖基化终产物食品奶粉为研究对象,借助细胞膜固相色谱技术和HPLC-MS/MS技术筛查其相应AGEs特异结合成分。结果表明:羧甲基赖氨酸(CML)、羧乙基赖氨酸(CEL)和吡咯素(Pyr)这3个成分是奶粉中AGEs主要成分,并测得奶粉样品中CML含量为23.38~32.68 mg/(kg样品);CEL含量为12.67~19.76 mg/(kg样品);Pyr含量为84.56~97.36 mg/(kg样品)。本方法操作简便、快速、特异性好,可应用于食品中AGEs成分的筛查。

Abstract: A method for the determination of advanced glycosylation end products in food was developed using cell membrane immobilized chromatography. The AGEs specific binding components of milk powder containing advanced glycosylation end products were screened by cell membrane immobilized chromatography and HPLC-MS/MS technology. The results showed that the three components of carboxymethyl-lysine,carboxyethyl-lysine and pyralline were the main components of AGEs in milk powder. The content of CML in the milk powder samples was 23.38~32.68 mg/(kg sample),and the content of CEL was 12.67~19.76 mg/(kg sample),and the content of Pyr was 84.56~97.36 mg/(kg sample). This method is simple,rapid and specific,and it is an effective method for the detection of advanced glycosylation end products in food.