Abstract: In this paper,quinoa was used as raw material and oil yield as test index,the optimum technological conditions of extraction of quinoa oil by supercritical CO₂ were determined by single factor experiments and orthogonal experiment,and the components were analyzed by gas chromatography(GC). The results showed that the optimum extraction conditions were as follows:extraction temperature 50℃,extraction pressure 30 MPa,extraction time 120 min. Under these conditions,quinoa oil yield was 4.134% and SD was 3.86×10^-5. A total of 26 compounds were isolated and identified from quinoa oil,among which linolenic acid,linoleic acid and oleic acid were the highest,with a relative content of them were 30.96%,21.94% and 15.23%,respectively. This study provided theoretical basis for comprehensive development and utilization and quality evaluation of quinoa.