Abstract: The antioxidant activity and hypoglycemic activity of Cyclocarya paliurus polysaccharides from different regions were preliminary evaluated taking the DPPH free radical scavenging capacity(DPPH·),hydroxyl radical(·OH),superoxide anion radical(O₂^-·)and alpha-glucosidase inhibitory activity as indexes. The results showed that,there were significant differences between the contents of Cyclocarya paliurus polysaccharides from different regions(p<0.05),in which Jiangxi Xiushui county was the highest 5.85%±0.02%,the Pingyang township in Rongjiang county of Guizhou was the lowest 3.50%±0.10%. The DPPH·,OH·,O₂^-·radical scavenging capacity and alpha-glucosidase inhibitory activities of polysaccharides from different habitats were different,and there was a certain dose effect relationship between the activity and the concentration of polysaccharides,that was,as the concentration of polysaccharide increased,the antioxidation and α-glucosidase inhibitory activity enhanced. The Cyclocarya paliurus polysaccharides had good antioxidant and hypoglycemic activity in vitro,which would be further exploited and utilized.