Abstract: To observe the protective effects of fibrinolytic enzyme of Lyophyllum ulmarium (LUFE) in rats with liver injury induced by lipopolysaccharide (LPS). The rats were randomly divided into five groups:the normal control, model, positive control, and the low-and high-dose LUFE groups.Rats in the low-and high-dose LUFE groups were i.g given LUFE at dose of 200 and 400 mg/kg for a period of 2 weeks.Besides the control group, other four groups were i.p given lipopolysaccharide to induce inflammatory liver injury in rats.The positive control rats were i.p given 5 mg/kg dexamethasone before the model establishment.The morphological changes of liver were observed by light microscope.The levels of TNF-α, IL-6, IL-1β, ALT, AST and ALB in serum were measured.The expression of TLR4, p-TAK1 and NF-κB proteins was detected by Western blot. The results showed that LUFE could reduce the inflammatory damage in liver tissue, decrease the serum levels of TNF-α, IL-6, IL-1β, ALT, AST, increased the serum ALB, and down-regulate the expression of TLR4, p-TAK1 and p-NF-κB proteins in rats with liver injury.The above results indicated that LUFE had protective effects on the liver injury in rats.The effects may be related to the regulation of TLR4-NF-κB signaling pathway.