Abstract: The pigment of Russula vinosa linolbl was thaken as material and extracted by ultrasonic-assisted extraction. The effects of variables on extraction yields of Russula vinosa linolbl pigment,such as ethanol concentration,liquid-solid ratio,extraction temperature and extraction time were investigated by single factor experiment. The response surface methodology was used to optimize the extraction conditions. Results showed that the optimum extraction conditions were as follows:ethanol concentration of 55%,ultrasonic time 29 min,extraction temperature 68 ℃and a material-liquid ratio of 1∶35 g/mL. The predicted absorbance of pigment extracts under the optimized conditions was 0.404,which was similar to the theoretical prediction value 0.406. Thus,the optimized pigment extraction conditions were reliable and feasible.