Abstract: Objective:Explore the possibility and significance of cultivation of Tricholoma giganteum with Mikania micrantha. Methods:The nutrient composition of M. micrantha was determined by conventional method. T. giganteum was cultivated by using M. micrantha as one of the substrate in T. giganteum culture medium, and the nutrient contents of the cultured T. giganteum were determined. Results:The contents of ash, total sugar, crude fiber, crude fat and crude protein of M. micrantha were 15.71%, 0.13%, 18.94%, 0.09% and 1.4% in dry weight, respectively, and the water content in fresh M. micrantha was 68.34%. There were not significant different in the completion time of mycelial growth (p>0.05), primordium formation time and harvest time between Formula 3 and Formula 4, but they have significantly difference from Formula 1 and Formula 2 (p ≤ 0.05). There were significantly different in the effective primordia and yield of Formulas 3 and Formula 4 (p ≤ 0.05), their effective primordia were 5.17, 9.47 no./bottle, and yield were 396.39, 718.29 g/bottle, respectively. The total sugar, crude fiber and ash content of T. giganteum cultivated with Formula 4 were 15.71%, 21.40%, 8.20%, respectively, they were higher than that of Formula 1, Formula 2 and Formula 3, while crude fat (1.90%) and crude protein contents (27.52%) were lower than them (p ≤ 0.05), which were 3.03%, 3.44%, 4.65% and 30.08%, 30.15%, 31.55%, respectively. The contents of potassium, sodium, magnesium and zinc of the T. giganteum cultivated in Formula 4 were 37447.5, 575.83, 1536.94, 83.38 mg/kg, respectively, and they were significantly higher than that of Formula 3, which were 35513.77, 406.92, 882.49, 69.26 mg/kg (p<0.05), while the contents of calcium (97.12 mg/kg) and iron (39.43 mg/kg) were lower than those of Formula 3, which were 497.07, 45.45 mg/kg (p<0.05), respectively. Conclusion:M. micrantha is a very good material for cultivating T. giganteum, with high contents of protein, calcium and iron. This result provides a theoretical basis for the further development and utilization of M. micrantha in T. giganteum cultivation, and obtains a new method for the comprehensive control of M. micrantha.