目的:研究菊苣多糖(chicory polysaccharide,CP)体外抗氧化能力及抗疲劳作用。方法:通过测定CP对羟自由基、超氧阴离子自由基、DPPH自由基的清除作用、还原力及其体外抗氧化能力,以小鼠耐力实验(负重游泳)和生化改变的检测评价CP的抗疲劳功效。结果:当浓度为2.4 mg/mL时,CP对羟自由基、超氧阴离子自由基及DPPH自由基清除率分别为40.93%、67.11%和58.17%,还原能力稍低于同浓度VC;与空白组相比,口服CP组小鼠负重游泳时间明显延长,且血尿素氮、血清乳酸和丙二醛水平显著降低(p<0.05),超氧化物歧化酶、肝糖原和肌糖原水平显著升高(p<0.05)。结论:CP具有较强的抗氧化能力和抗疲劳作用。
Objective:To study the in vitro antioxidant activities and anti-fatigue effect of chicory polysaccharide. Methods:The scavenging activity of CP against hydroxyl radical, superoxide anion radical, superoxide anion radical and its reduction force were measured to evaluate their antioxidant activities in vitro. The anti-fatigue effect of CP was evaluated by endurance test (weight-bearing swimming)and biochemical indicators changes in mice. Results:At the concentration of 2.4 mg/mL, the hydroxyl radical, superoxide anion free radical and DPPH free radical scavenging rates of CP were 40.93%, 67.11%, 58.17%, respectively, while the reducing capacity of CP was slightly lower than the same concentration of VC. Compared with the blank control group, the swimming time of CP-treated mice was prolonged, and the levels of blood urea nitrogen, serum lactic acid, and malondialdehyde were significantly decreased (p<0.05), superoxide dismutase (SOD), hepatic glycogen (HG)and muscle glycogen (MG)levels were significantly increased (p<0.05). Conclusion:CP had strong antioxidant and anti fatigue effect.