目的:确定陈皮总黄酮提取的最佳工艺条件,并初步测试其抑菌活性。方法:采用微波辅助乙醇法提取陈皮总黄酮,依据单因素实验建立BBD实验模型,用响应面优化提取工艺,并进行验证实验;黄酮提取液经HP-20型大孔树脂分离提纯后,采用滤纸片法测试其对大肠杆菌、枯草芽孢杆菌及金黄色葡萄球菌的抑菌活性。结果:经验证,陈皮总黄酮在乙醇体积浓度60%、微波功率619 W、微波时间3 min、提取温度62 ℃、料液比1:21 (g/mL)时的黄酮得率最高,为1.3468%±0.0142%,与预测值(1.3575%)的偏差仅为0.0107%,说明该模型方程与实际拟合良好。抑菌活性实验结果表明:陈皮黄酮对被测菌种均有一定的抑菌活性,其中对大肠杆菌和金黄色葡萄球菌的抑菌效果优于对照茶多酚。
Objective:To determine the best extraction conditions of the total flavonoids from tangerine peel,and preliminarily test of its antibacterial activity. Methods:The total flavonoids of tangerine peel(FTP)were extracted with ethanol through microwave-assisted method,and the Box-Behnken Design(BBD)experiment model was established according to the single factor experiments.And the results were validated by experimental verification.The FTP was purified by HP-20 macroporous resin. Then the antibacterial activity against E. enteritidis,Bacillus subtilis and Staphylococcus aureus was tested by filter paper method. Conclusion:The extraction results showed that yield of flavonoids was the highest 1.3468%±0.0142% when the concentration of ethanol was 60%,the microwave power was 619 W,the microwave time was 3 min,the extraction temperature was 62 ℃ and material to solvent ratio was 1:21 (g/mL),which had been verified by experiments. The deviation of the result to predicted value(1.3575%)was only 0.0107%,which indicated that the model and the actual fitted well.The antibacterial activity results showed that:FTP had different antibacterial activity against the tested bacterias,which was more effective against E. enteritidis and Staphylococcus aureus than the control drug tea polyphenols.