Abstract: The content and nutrition assessment of 17 amino acids of purple passionfruit and other 5 fruits were investigated. The content order of total amino acids(TAA)was:purple passionfruit>banana>pitaya>mango>orange>papaya. The content order of essential amino acids(EAA)was:banana>purple passionfruit>pitaya>mango>papaya>orange. The first limiting amino acid of 6 fruits was methionine(Met). The top 3 content amino acids were aspartic acid,proline and glutamic acid. The highest EAA of purple passionfruit was lysine(26.6%). Purple passionfruit had the highest content of sour and MSG-like amino acids(SMAA),sweet amino acids and bitter amino acids in 6 fruits. Aspartic acid(Asp)and glutamic acid(Glu)had high content in amino acids,which played an important role in the MSG-like flavor of purple passionfruit. Passionfruit was rich in flavor.