Abstract: The effects of different fermentation times, treatment methods and treatment solutions of the antagonistic bacterium Burkholderia contaminans B-1 on the postharvest storage of strawberry fruit were studied by natural decay and wound inoculation method. And the safety evaluation of the antagonistic bacterium B-1 including acute oral toxicity test on rats, skin, eyes irritation test on healthy rabbits and dermal allergy reaction on guinea pigs were performed. The results showed that inhibition rate of 24 hours culturing, 3 minutes immersing and with dilution five times of the fermented liquid were decreased by 55.04%, 46.74%, 39.98% respectively compared with the CK. The inhibition rate of gray mold on strawberry fruit reached 100% in the treatment of fermentation solution, diluted 5 times fermentation broth and bacterial suspension. The antagonistic bacteria in rats with acute LD₅₀>5000 mg/kg. The mean index of skin irritation and eye irritation(I.A.O.I)on rabbits were 0. The mean index of eye irritation(M.I.O.I)were 0 after 48 hours. Sensitization rate of skin sensitization(sensitization)test was 0. The result indicated that the bacterium is safe as a biocontrol bacterium. This study laid the foundation for the productive application of the biocontrol bacterium.