Abstract: The yeast Meyerozyma pulcherrima(M. pulcherrima)was evaluated for their effectiveness against Colletotrichum gloeosporioides(C. gloeosporioides)in the mango by in vitro experiment. The results showed that M. pulcherrima had a significant bacteriostatic effect on C. gloeosporioides in YPD medium. M. pulcherrima showed a high antagonistic effect in mangos, with significant inhibition of 57.81% and 40.87% of anthracnose at 15℃ and 25℃, respectively. At the same time, the possible action mechanisms involved in the inhibition was studied, Scanning electron microscopy showed that yeast could produce biofilm, it could inhibit the invasion of C. gloeosporioides. M. pulcherrima could secrete chitinase and β-1, 3-glucanase, which had a role of inhibiting pathogens. Antagonistic yeast could also traceably absorb iron of surrounding environment to increase the effects of inhibiting the growth of pathogens.