Abstract: Objective:To study the effect of black wolfberry extracts against hepatitis B virus and to detect its antioxidant activity. Methods:HepG22.2.15 cells were cultured in vitro, MTT was used to detect the cytotoxicity of black wolfberry extracts against HepG2 2.2.15 cells. The expression of HBsAg and HBeAg in the culture medium of HepG2 2.2.15 cells was detected by double antibody sandwich method. To determine the reducing prower and scavenging capacity of hydroxyl radical and DPPH free radical of different concentration of black wolfberry.Results:MTT assay showed no obvious cytotoxicity of black wolfberry extracts on HepG2 2.2.15 cells. Compared with the negative control group, black wolfberry extracts had inhibitory effects on the expression of HBsAg and HBeAg in HepG2 2.2.15 cells(p<0.05).And with the increase of the concentration of black wolfberry extracts, its abilities of reducing and scavenging hydroxyl radical, scavenging DPPH free radical(DPPH·)were also gradually enhanced.Conclusion:black wolfberry extracts have inhibitory effect on hepatitis B virus, and have a good antioxidant capacity.