Abstract: Objective: To study the antioxidant activity of flavonoids from jujube leaves extract in aging mice's heart liver and serum. Methods: flavonoids from Jujube leaves was extracted with ethanol, the content of total flavonoids in jujube leaves was determined and its antioxidant activity was determined. The aging mouse model induced by subcutaneous injection of D-galactose was orally administered with Jujube leaves extract. The content of malondiaidehyde ( MDA) , the activities of superoxide dismutase ( SOD) and glutathione peroxidase ( GSH-Px) and total antioxidant capacity ( T-AOC) in heart, liver and serum of the mice were assessed; do HE staining slice, observe the state of tissue cells.Results: The content of total flavonoids in leaves was ( 18.60 ± 1.06) mg/g, The IC50 values of DPPH free radicals, hydroxyl radicals, ABTS radicals, and superoxide anion radicals were 0.997, 1.035, 1.039, 0.983 mg/m L, all with positive control group had significant difference; D-galactose could obviously reduce T-AOC as well as SOD and GSH-Px activities, and enhance MDA contents in mouse heart, liver and serum, there was significant difference between the blank control group and the model group.In contrast, flavonoids from Jujube leaves could significantly enhance T-AOC, and SOD and GSH-Px activities, and reduce MDA contents in serum, and cerebrum, heart and liver tissues of the aging mice, especially in serum; According to the observation of pathological, flavonoids of jujube leaf have antioxidation ability. Conclusion: flavonoids from Jujube leaves could improve the activities of endogenous antioxidantenzymes in aging mice.Therefore, it has good antioxidant and anti-aging effects.