反相高效液相色谱测定饮料中新橙皮苷二氢查尔酮
Determination of neohesperidin dihydrochalcone in beverages by reversed phase high performance liquid chromatography
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摘要: 建立了反相高效液相色谱检测饮料中新橙皮苷二氢查尔酮的分析方法。分别对提取方法、沉淀剂的使用和色谱条件进行了优化。采用核壳型的Accucore C18(2.6μm,4.6 mm×100 mm)色谱柱为分析柱,以甲醇和水(45∶55,v/v)为流动相,等度洗脱,流速1.0 m L/min,柱温25℃,紫外检测波长283 nm,进样量10μL,外标法定量分析。结果表明新橙皮苷二氢查尔酮的分离在10 min以内,具有良好峰形,在0.5~50 mg/L浓度范围内,有良好的线性关系,相关系数r>0.999,检出限为0.15 mg/L,在10、40、80 mg/kg加标水平下平均回收率在90.2%~103.5%之间,相对标准偏差(RSD)在1.96%~7.59%之间(n=6)。结果表明该方法选择性强、灵敏度高、操作简便,可用于饮料中新橙皮苷二氢查尔酮的检测。Abstract: A method of reversed phase high performance liquid chromatography was established for the determination of neohesperidin dihydrochalcone in beverages.The extraction method, precipitant and chromatography conditions were optimized.The separation was performed on an Accucore C18 ( 2.6 μm, 4.6 mm × 100 mm) with methanol and water ( 45∶ 55, v/v) isocratic elution as the mobile phase at a flow rate of 1.0 m L/min and 25 ℃.The detection wavelength was at 283 nm and the sample injection volume was 10 μL. Neohesperidin dihydrochalcone was isolated with satisfactory resolution within 10 min. The concentration of neohesperidin dihydrochalcone in the range of 0.5 ~ 50 mg/L was linearly correlated to the peak area, with correlation coefficients higher than 0.999 and detection limit of 0.15 mg/L.The recoveries of neohesperidin dihydrochalcone at three spiked levels ( 10, 40, 80 mg/kg) were in the range of 90.2% 103.5% and the relative standard deviations ( RSDs) were in the range of 1.96% ~ 7.59% ( n = 6) . The method is specific, sensitive, simple and suitable for the determination of neohesperidin dihydrochalcone in beverages.