Abstract: Objective: A quantitative method for simultaneous detection of aflatoxin B₁, B₂, G₁, G₂ and ochratoxin A in 20 batches of edible oil was carried out by high performance liquid chromatography. Methods: After the sample was vortexed and centrifuged, the target toxin was purified by multifunctional purification column, using SHIMADZU Inertsil ODS-C₁₈ column ( 4.6 mm × 150 mm, 5 μm) , FLD detector and methanol ( A) /0.5% acetic acid water ( B) by gradient elution, the injection volume was 10 μL, flow rate was 0.8 m L/min, column temperature at 35 ℃, then wavelength fluorescence was changed to detect aflatoxin B₁, B₂, G₁, G₂, ochratoxin A and study on methodology.Results: The linear ranges of AFB₁, AFG₁, AFB₂, AFG₂ and OTA were 0.25 ~ 40.6 ng/m L ( r = 0.9999) , 0.5 ~ 40.4 ng/m L ( r = 0.9997) , 0.06 ~ 10.06 ng/m L ( r = 0.9999) , 0.06 ¹0.08 ng/m L ( r = 0.9999) and 1.01⁵0.5 ng/m L ( r = 1.00) .The average recovery rate of five mycotoxins was 91.40% ~ 109.4%.The results showed that 4 batches of 20 samples of edible oil were positive and tested by aflatoxin G₁ and G₂. Conclusion: The method is accurate and rapid, and can be widely used for the quantitative detection of aflatoxin B₁, B₂, G₁, G₂ and ochratoxin A in edible oil.