Abstract: The strains of lactobacillus paracasei VL8 was isolated form viili as base as described previously, futher researching of the immunomodulation of VL8-EPS was investigated. Firstly, preparation of mouse spleen lymphocytes and the proliferation of spleen lymphocytes in mice with different concentrations of VL8-EPS were determined by MTT.The contents of IL-2, IFN-γand IL-6 were determined by using ELISA.The results showed that VL8-EPS at a concentration ranging from 12.5 μg/m L to800 μg/m L resulted in a significant increase of spleen lymphocyte proliferation, VL8-EPS could also enhance the effect of Con A-induced spleen lymphocyte proliferation and the induction effects was higher than that of single application of VL8-EPS.Moreover, VL8-EPS could significantly increase the secretion level of IL-2, IFN-γ and IL-6 in Con A-induced spleen lymphocyte. In summary, VL8-EPS can effectively increase spleen lymphocyte proliferation and enhance the effect of Con Ainduced spleen cell growth. VL8-EPS has the ability to enhance immune responses by increasing the secretion of cytokine. It showed that VL8-EPS could regulate the immune mechanisms partly.