Abstract: In order to improve the yield, based on the single factor experiments, the response surface methodology was employed to optimize the extraction conditions of proanthocynidins from Lycium ruthenicum Murr.. The process of quadric polynomial mathematics model was established by investigating the effect of ethanol concentration, extraction temperature, extraction time and their interaction on proanthocyanidins.Experiments showed that regression equation fit well with experimental data and the effect of investigated parameters were extremely significant. The optimal of extraction conditions including ethanol concentration, extraction temperature, extraction time were 56%, 46 ℃ and 48 min respectively. The content of proanthocyanidins extracted from Lycium ruthenicum Murr.under the conditions was 4.315%.Compared with the predicted value of response surface, the error was 1.5%, which indicates that it is suitable for proanthocyanidins extracts from Lycium ruthenicum Murr.