模拟常温物流下大菱鲆菌相变化规律与优势腐败菌鉴定

唐荣 张彩丽 孙博文 刘尊英

唐荣, 张彩丽, 孙博文, 刘尊英. 模拟常温物流下大菱鲆菌相变化规律与优势腐败菌鉴定[J]. 食品工业科技, 2017, (05): 334-339. doi: 10.13386/j.issn1002-0306.2017.05.055
引用本文: 唐荣, 张彩丽, 孙博文, 刘尊英. 模拟常温物流下大菱鲆菌相变化规律与优势腐败菌鉴定[J]. 食品工业科技, 2017, (05): 334-339. doi: 10.13386/j.issn1002-0306.2017.05.055
TANG Rong, ZHANG Cai-li, SUN Bo-wen, LIU Zun-ying. Changes of bacterial flora composition and identification of the dominant spoilage bacteria in turbot under simulated of room temperature logistics[J]. Science and Technology of Food Industry, 2017, (05): 334-339. doi: 10.13386/j.issn1002-0306.2017.05.055
Citation: TANG Rong, ZHANG Cai-li, SUN Bo-wen, LIU Zun-ying. Changes of bacterial flora composition and identification of the dominant spoilage bacteria in turbot under simulated of room temperature logistics[J]. Science and Technology of Food Industry, 2017, (05): 334-339. doi: 10.13386/j.issn1002-0306.2017.05.055

模拟常温物流下大菱鲆菌相变化规律与优势腐败菌鉴定

doi: 10.13386/j.issn1002-0306.2017.05.055
基金项目: 

国家科技型中小企业科技技术创新基金(14C26213712172);

详细信息
    作者简介:

    唐荣 (1992-) , 女, 硕士生, 研究方向:水产品高值化利用, E-mail:956336816@qq.com。;

    刘尊英 (1974-) , 女, 博士, 副教授, 研究方向:水产品保鲜与加工, E-mail:naturefoods@163.com。;

  • 中图分类号: TS254.1

Changes of bacterial flora composition and identification of the dominant spoilage bacteria in turbot under simulated of room temperature logistics

  • 摘要: 以大菱鲆为研究对象,将传统选择性培养基法和16S r DNA序列分析法相结合,并通过系统发育树分析,探索模拟常温物流下大菱鲆的菌相变化规律及优势腐败菌。结果表明:模拟常温物流下,传统分析与16S r DNA序列分析均表明,大菱鲆初始菌相与贮藏末期菌相明显不同。经16S r DNA序列、NCBI数据库比对以及系统发育树的构建表明,大菱鲆末期菌相主要有气单胞菌(Ameromonas sp.)、肠杆菌(Enterobacteriaceae sp.)、红杆菌(Rhodobacteraceae sp.)、发光杆菌(Photo bacterium sp.)、变形杆菌(Gammaproteobacteria sp.)、希瓦氏菌(Shewanella sp.)、拟杆菌(Bacteroidales sp.)和腈基降解菌(Nitriliruptoraceae sp.),其中气单胞菌(Aeromonas sp.)和肠杆菌中的柠檬酸杆菌(Citrobacter sp.)和哈夫尼氏菌(Hafnia sp.)为大菱鲆模拟常温物流下的优势腐败菌。 
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  • 收稿日期:  2016-07-12

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