表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

李莹; 钟金钢; 马骁; 李仕萍; 齐攀

doi:10.13386/j.issn1002-0306.2017.05.049

表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

doi: 10.13386/j.issn1002-0306.2017.05.049

李莹^1,2,
钟金钢^1,3,
马骁^1,3,
李仕萍^1,3,
齐攀⁴

1. 光电信息与传感技术广东普通高校重点实验室暨南大学
2. 暨南大学预科部
3. 暨南大学光电工程系
4. 广东交通职业技术学院电子工程系

基金项目:

国家自然科学基金项目(61605063和41206081)；广东省自然科学基金项目(2014A030310483和2015A030310458)；广东省高等职业院校珠江学者岗位计划资助项目(2016年度)；

详细信息

作者简介:
李莹 (1976-) , 女, 博士, 教授, 主要从事生物医学信息技术研究, E-mail:916407691@qq.com。;

齐攀 (1982-) , 男, 博士, 副教授, 主要从事光电检测技术方面的研究, E-mail:82381299@qq.com。;

中图分类号: TS207.3
计量
- 文章访问数: 119
- HTML全文浏览量: 10
- PDF下载量: 264
- 被引次数: 0
出版历程
- 收稿日期: 2016-08-26

Research of the surface plasmon resonance immunosensor for rapid detection of microcystin

LI Ying^1,2,
ZHONG Jin-gang^1,3,
MA Xiao^1,3,
LI Shi-ping^1,3,
QI Pan⁴

Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University
Pre-university, Jinan University
Department of Optoelectronic Engineering, Jinan University
Department of Electronics Engineering, Guangdong Communication Polytechnic

摘要

摘要: 针对现有微囊藻毒素（MC-LR）检测方法操作复杂、因标记而污染环境、仪器贵重,不利于现场快速检测等问题,将自行研制的表面等离子体共振（SPR）生物芯片检测仪应用于微囊藻毒素的检测,提出抑制型SPR生物芯片快速检测痕量微囊藻毒素的方法。采用该方法分别对浓度为3.5、2.5、1.5、1、0μg/L的MC-LR样品进行了检测。结果表明:该方法检测限小于1μg/L,可满足世界卫生组织（WHO）对于饮用水和我国地表水环境质量标准中MC-LR最低含量检测的需求。该方法完成一个样品检测耗时约8 min,相比于高效液相色谱法（HPLC）和酶联免疫吸附法（ELISA）等传统检测方法,快速定量是其最大的优势,可用于食品质量监控和现场实时检测。
- 表面等离子体共振 /
- 生物芯片 /
- 无标记快速检测 /
- 微囊藻毒素
Abstract: As the existing pesticide detection methods were complicated, would pollute the environment by marking, with precious instrument, and not conducive to on-site rapid detection, the self-developed surface plasmon resonance biological chip detector was used in the detection of microcystin ( MC-LR) , a method for rapid detection of microcystin by inhibiting type SPR biological chip was proposed.The competitive inhibition immunoassay was preceded in MC-LR with concentrations of 3.5, 2.5, 1.5, 1, 0 μg/L.The experimental results showed that the detection limit could be 1 μg/L which could meet the needs of the World Health Organization ( WHO) for detecting the minimum content of MC-LR in drinking water and surface water environmental quality standards in China.The detection of 1 sample would take about 8 min by this method, compared with the traditional methods such as high performance liquid chromatography ( HPLC) and enzyme linked immunosorbent assay ( ELISA) , the rapid and quantitative was the biggest advantage of this method.The established device and method could be used for food quality monitoring and on-site real-time detection.
- surface plasmon resonance /
- biochip /
- label free rapid detection /
- microcystin

HTML全文

参考文献(19)

[1]	Carmichael W W.The Cyanotoxins[J].Advances in Botanical Research, 1997, 27:211-256.
[2]	徐潇颖, 刘柱, 梁晶晶, 等.全自动固相萃取-高效液相色谱法同时测定饮用水中3种微囊藻毒素[J].食品工业科技, 2016, 37 (18) :60-62.
[3]	Arzate-Cárdenas M A, Olvera-Ramírez R, MartínezJerónimo F.Microcystis, toxigenic strains in urban lakes:a case of study in Mexico City[J].Ecotoxicology, 2010, 19 (19) :1157-1165.
[4]	Peng F, Xue H H, Chen W C, et al.Investigation on simultaneous determination of deltamethrin, carbaryl, microcystin-LR and benzo[a]pyrene by online SPE-HPLC method[J].Advanced Materials Research, 2014, 1073-1076:575-578.
[5]	梁丽丽, 弓爱君, 李红梅, 等.高效液相色谱法检测水体中微囊藻毒素[J].分析化学, 2010, 38 (5) :740-742.
[6]	周宏敏, 欧惠超, 任鹏, 等.利用SPR技术测定湖水中微囊藻毒素[J].中国环境科学, 2012, 32 (7) :1284-1287.
[7]	Yamamura S, Bartram J, Csanady M, et al.Drinking water Guidelines and standards[C].World Health Organization, Geneva, Switzerland, 2004.
[8]	Yu T, Xie P, Dai M, et al.Determinations of MC-LR and[Dha7]MC-LR concentrations and physicochemical properties by liquid chromatography-tandem mass spectrometry[J].Bulletin of Environmental Contamination&Toxicology, 2009, 83 (5) :757-760.
[9]	Nagata S.Development and application of highly sensitive anti-immune complex ELISAs for microcystins in tap water[J].Food&Agricultural Immunology, 2010, 12 (3) :231-241.
[10]	Li S P, Zhong J G.Simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging by use of digital holography[J].Biomedical Optics Express, 2012, 3 (12) :3190-3202.
[11]	Espiritu R A, Matsumori N, Murata M, et al.Interaction between the marine sponge cyclic peptide theonellamide a and sterols in lipid bilayers as viewed by surface plasmon resonance and solid-state 2H nuclear magnetic resonance[J].Biochemistry, 2013, 52 (14) :2410-2418.
[12]	Homola J, Lu H B, Yee S S.Dual-channel surface plasmon resonance sensor with spectral discrimination of sensing channels using dielectric overlayer[J].Electronics Letters, 1999, 35 (13) :1105-1106.
[13]	Nelson B P, Grimsrud T E, Liles M R, et al.Surface plasmon resonance imaging measurements of DNA and RNA hybridization adsorption onto DNA microarrays[J].Analytical Chemistry, 2001, 73 (1) :1-7.
[14]	Yuk J S, Kim H S, Jung J W, et al.Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging[J].Biosensors and Bioelectronics, 2006, 21 (8) :1521-1528.
[15]	齐攀, 钟金钢, 李莹, 等.用于食品安全检测的便携式表面等离子体共振生物传感器[J].光电工程, 2014 (3) :22-27.
[16]	Schasfoort R B M, Tudos A J.Handbook of surface plasmon resonance[M].Royal Society of Chemistry, 2008.
[17]	Karlsson R.SPR for molecular interaction analysis:a review of emerging application areas[J].Journal of Molecular Recognition, 2004, 17 (3) :151-161.
[18]	王晓萍, 詹舒越, 罗昭锋, 等.地表水微囊藻毒素的表面等离波子共振免疫检测方法研究[J].光学学报, 2012, 32 (2) :0212005-1-0212005-6.
[19]	郭伟, 黑亮, 杨燕婷, 等.固相萃取-高效液相色谱法优化测定饮用水中的微囊藻毒素[J].广东农业科学, 2014, 1:146-148.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 119
HTML全文浏览量: 10
PDF下载量: 264
被引次数: 0

表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

doi: 10.13386/j.issn1002-0306.2017.05.049

作者简介: 李莹 (1976-) , 女, 博士, 教授, 主要从事生物医学信息技术研究, E-mail:916407691@qq.com。; 齐攀 (1982-) , 男, 博士, 副教授, 主要从事光电检测技术方面的研究, E-mail:82381299@qq.com。;

计量

出版历程

Research of the surface plasmon resonance immunosensor for rapid detection of microcystin

计量

出版历程

目录

作者简介:
李莹 (1976-) , 女, 博士, 教授, 主要从事生物医学信息技术研究, E-mail:916407691@qq.com。;

齐攀 (1982-) , 男, 博士, 副教授, 主要从事光电检测技术方面的研究, E-mail:82381299@qq.com。;