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中国精品科技期刊2020

表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

李莹 钟金钢 马骁 李仕萍 齐攀

李莹, 钟金钢, 马骁, 李仕萍, 齐攀. 表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究[J]. 食品工业科技, 2017, (05): 304-307. doi: 10.13386/j.issn1002-0306.2017.05.049
引用本文: 李莹, 钟金钢, 马骁, 李仕萍, 齐攀. 表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究[J]. 食品工业科技, 2017, (05): 304-307. doi: 10.13386/j.issn1002-0306.2017.05.049
LI Ying, ZHONG Jin-gang, MA Xiao, LI Shi-ping, QI Pan. Research of the surface plasmon resonance immunosensor for rapid detection of microcystin[J]. Science and Technology of Food Industry, 2017, (05): 304-307. doi: 10.13386/j.issn1002-0306.2017.05.049
Citation: LI Ying, ZHONG Jin-gang, MA Xiao, LI Shi-ping, QI Pan. Research of the surface plasmon resonance immunosensor for rapid detection of microcystin[J]. Science and Technology of Food Industry, 2017, (05): 304-307. doi: 10.13386/j.issn1002-0306.2017.05.049

表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

doi: 10.13386/j.issn1002-0306.2017.05.049
基金项目: 

国家自然科学基金项目(61605063和41206081); 广东省自然科学基金项目(2014A030310483和2015A030310458); 广东省高等职业院校珠江学者岗位计划资助项目(2016年度);

详细信息
    作者简介:

    李莹 (1976-) , 女, 博士, 教授, 主要从事生物医学信息技术研究, E-mail:916407691@qq.com。;

    齐攀 (1982-) , 男, 博士, 副教授, 主要从事光电检测技术方面的研究, E-mail:82381299@qq.com。;

  • 中图分类号: TS207.3

Research of the surface plasmon resonance immunosensor for rapid detection of microcystin

  • 摘要: 针对现有微囊藻毒素(MC-LR)检测方法操作复杂、因标记而污染环境、仪器贵重,不利于现场快速检测等问题,将自行研制的表面等离子体共振(SPR)生物芯片检测仪应用于微囊藻毒素的检测,提出抑制型SPR生物芯片快速检测痕量微囊藻毒素的方法。采用该方法分别对浓度为3.5、2.5、1.5、1、0μg/L的MC-LR样品进行了检测。结果表明:该方法检测限小于1μg/L,可满足世界卫生组织(WHO)对于饮用水和我国地表水环境质量标准中MC-LR最低含量检测的需求。该方法完成一个样品检测耗时约8 min,相比于高效液相色谱法(HPLC)和酶联免疫吸附法(ELISA)等传统检测方法,快速定量是其最大的优势,可用于食品质量监控和现场实时检测。 
  • [1] Carmichael W W.The Cyanotoxins[J].Advances in Botanical Research, 1997, 27:211-256.
    [2] 徐潇颖, 刘柱, 梁晶晶, 等.全自动固相萃取-高效液相色谱法同时测定饮用水中3种微囊藻毒素[J].食品工业科技, 2016, 37 (18) :60-62.
    [3] Arzate-Cárdenas M A, Olvera-Ramírez R, MartínezJerónimo F.Microcystis, toxigenic strains in urban lakes:a case of study in Mexico City[J].Ecotoxicology, 2010, 19 (19) :1157-1165.
    [4] Peng F, Xue H H, Chen W C, et al.Investigation on simultaneous determination of deltamethrin, carbaryl, microcystin-LR and benzo[a]pyrene by online SPE-HPLC method[J].Advanced Materials Research, 2014, 1073-1076:575-578.
    [5] 梁丽丽, 弓爱君, 李红梅, 等.高效液相色谱法检测水体中微囊藻毒素[J].分析化学, 2010, 38 (5) :740-742.
    [6] 周宏敏, 欧惠超, 任鹏, 等.利用SPR技术测定湖水中微囊藻毒素[J].中国环境科学, 2012, 32 (7) :1284-1287.
    [7] Yamamura S, Bartram J, Csanady M, et al.Drinking water Guidelines and standards[C].World Health Organization, Geneva, Switzerland, 2004.
    [8] Yu T, Xie P, Dai M, et al.Determinations of MC-LR and[Dha7]MC-LR concentrations and physicochemical properties by liquid chromatography-tandem mass spectrometry[J].Bulletin of Environmental Contamination&Toxicology, 2009, 83 (5) :757-760.
    [9] Nagata S.Development and application of highly sensitive anti-immune complex ELISAs for microcystins in tap water[J].Food&Agricultural Immunology, 2010, 12 (3) :231-241.
    [10] Li S P, Zhong J G.Simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging by use of digital holography[J].Biomedical Optics Express, 2012, 3 (12) :3190-3202.
    [11] Espiritu R A, Matsumori N, Murata M, et al.Interaction between the marine sponge cyclic peptide theonellamide a and sterols in lipid bilayers as viewed by surface plasmon resonance and solid-state 2H nuclear magnetic resonance[J].Biochemistry, 2013, 52 (14) :2410-2418.
    [12] Homola J, Lu H B, Yee S S.Dual-channel surface plasmon resonance sensor with spectral discrimination of sensing channels using dielectric overlayer[J].Electronics Letters, 1999, 35 (13) :1105-1106.
    [13] Nelson B P, Grimsrud T E, Liles M R, et al.Surface plasmon resonance imaging measurements of DNA and RNA hybridization adsorption onto DNA microarrays[J].Analytical Chemistry, 2001, 73 (1) :1-7.
    [14] Yuk J S, Kim H S, Jung J W, et al.Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging[J].Biosensors and Bioelectronics, 2006, 21 (8) :1521-1528.
    [15] 齐攀, 钟金钢, 李莹, 等.用于食品安全检测的便携式表面等离子体共振生物传感器[J].光电工程, 2014 (3) :22-27.
    [16] Schasfoort R B M, Tudos A J.Handbook of surface plasmon resonance[M].Royal Society of Chemistry, 2008.
    [17] Karlsson R.SPR for molecular interaction analysis:a review of emerging application areas[J].Journal of Molecular Recognition, 2004, 17 (3) :151-161.
    [18] 王晓萍, 詹舒越, 罗昭锋, 等.地表水微囊藻毒素的表面等离波子共振免疫检测方法研究[J].光学学报, 2012, 32 (2) :0212005-1-0212005-6.
    [19] 郭伟, 黑亮, 杨燕婷, 等.固相萃取-高效液相色谱法优化测定饮用水中的微囊藻毒素[J].广东农业科学, 2014, 1:146-148.
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  • 收稿日期:  2016-08-26

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