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中国精品科技期刊2020

保加利亚乳杆菌D-乳酸脱氢酶同工酶基因在大肠杆菌中的表达

黄艳娜 刘振民 游春苹

黄艳娜, 刘振民, 游春苹. 保加利亚乳杆菌D-乳酸脱氢酶同工酶基因在大肠杆菌中的表达[J]. 食品工业科技, 2017, (05): 159-163. doi: 10.13386/j.issn1002-0306.2017.05.021
引用本文: 黄艳娜, 刘振民, 游春苹. 保加利亚乳杆菌D-乳酸脱氢酶同工酶基因在大肠杆菌中的表达[J]. 食品工业科技, 2017, (05): 159-163. doi: 10.13386/j.issn1002-0306.2017.05.021
HUANG Yan-na, LIU Zhen-min, YOU Chun-ping. Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli[J]. Science and Technology of Food Industry, 2017, (05): 159-163. doi: 10.13386/j.issn1002-0306.2017.05.021
Citation: HUANG Yan-na, LIU Zhen-min, YOU Chun-ping. Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli[J]. Science and Technology of Food Industry, 2017, (05): 159-163. doi: 10.13386/j.issn1002-0306.2017.05.021

保加利亚乳杆菌D-乳酸脱氢酶同工酶基因在大肠杆菌中的表达

doi: 10.13386/j.issn1002-0306.2017.05.021
基金项目: 

国家“十二五”科技支撑计划(2013BAD18B01,2013BAD18B02); 上海市闵行区领军人才项目(201443); 上海科学技术委员会项目(16DZ2280600);

详细信息
    作者简介:

    黄艳娜 (1980-) , 女, 博士, 工程师, 研究方向:益生菌代谢工程及代谢调控, E-mail:huangyanna@brightdairy.com。;

    游春苹 (1982-) , 女, 博士, 高级工程师, 研究方向:益生菌代谢工程及代谢调控, E-mail:youchunping@brightdairy.com。;

  • 中图分类号: TS201.3

Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli

  • 摘要: 通过对保加利亚乳杆菌ATCC11842的D-乳酸脱氢酶基因的克隆,分离获得了ldb0101、ldb0813、ldb1010、ldb1147和ldb2021五种同工酶基因,并分别构建重组质粒转化大肠杆菌JM109,实现D-LDH同工酶基因的表达。结合重组大肠杆菌的摇瓶发酵实验和D-乳酸的合成,初步确定D-乳酸脱氢酶Ldb0101和Ldb1010在保加利亚乳杆菌中对D-乳酸的合成起着重要的作用。 
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  • 收稿日期:  2016-09-06

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