Abstract: The amygdalin content in almond from seven provenances in Inner Mongolia were analyzed by high performance liquid chromatography( HPLC).The results showed that the amygdalin content of bitter almond from different provenances had significant differences( p < 0.05). The average amygdalin content among provenances was 5.43%,content range for 4.34% ~6.25%.The average coefficient of variation was 0.07. Jarud Banner had the higher coefficient of variation than other sources.Amygdalin content had significantly negative correlation with latitude and longitude( 0.806,0.722),and significantly positive correlation with altitude( 0.896). Cluster analysis divided seven provenances into 2 classes,clustering results were consistent with the correlation results.The results of the study provide a theoretical basis for the deep processing and medicinal value of apricot in Inner Mongolia.