HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态

熊珺 覃毅磊 龚亮 赖毅东

熊珺, 覃毅磊, 龚亮, 赖毅东. HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态[J]. 食品工业科技, 2017, (04): 67-72. doi: 10.13386/j.issn1002-0306.2017.04.004
引用本文: 熊珺, 覃毅磊, 龚亮, 赖毅东. HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态[J]. 食品工业科技, 2017, (04): 67-72. doi: 10.13386/j.issn1002-0306.2017.04.004
XIONG Jun, QIN Yi-lei, GONG Liang, LAI Yi-dong. Simultaneous quantification of the speciation of inorganic selenium and Se amino acids in food by high performance liquid chromatography combined with inductively coupled plasma mass spectrometry[J]. Science and Technology of Food Industry, 2017, (04): 67-72. doi: 10.13386/j.issn1002-0306.2017.04.004
Citation: XIONG Jun, QIN Yi-lei, GONG Liang, LAI Yi-dong. Simultaneous quantification of the speciation of inorganic selenium and Se amino acids in food by high performance liquid chromatography combined with inductively coupled plasma mass spectrometry[J]. Science and Technology of Food Industry, 2017, (04): 67-72. doi: 10.13386/j.issn1002-0306.2017.04.004

HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态

doi: 10.13386/j.issn1002-0306.2017.04.004
基金项目: 

广东省质量技术监督局科技项目(2013ZS01);

详细信息
    作者简介:

    熊珺(1978-),女,博士,高工,主要从事痕量化合物检测技术方面的研究,E-mail:xj@gddqt.com。;

  • 中图分类号: TS207.3;O657.63

Simultaneous quantification of the speciation of inorganic selenium and Se amino acids in food by high performance liquid chromatography combined with inductively coupled plasma mass spectrometry

  • 摘要: 以亚硒酸盐(Se(Ⅳ))、硒酸盐(Se(Ⅵ))、硒胱氨酸(Se Cys2)、硒蛋氨酸(Se Met)、甲基硒代半胱氨酸(Me Se Cys)和硒代乙硫氨酸(Se Et)为硒形态的目标分析物,采用Dionex Ion Pac AS11色谱柱(250 mm×4.0 mm)为分离柱,通过优化流动相的p H、浓度、甲醇含量、流速和色谱柱的柱温等因素对六种目标硒形态分离及不同提取方法对目标分析物提取效率的影响,建立了高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)在线联用同时测定食品中无机硒和硒氨基酸的分析方法。Me Se Cys、Se Met、Se Et、Se(Ⅳ)、Se Cys2和Se(Ⅵ)的检出限分别为0.25、0.20、0.35、0.15、0.30、0.15μg Se/L;Me Se Cys和Se Cys2的线性范围为2.02500μg Se/L,Se(Ⅳ)和Se(Ⅵ)线性范围为1.21200μg Se/L,Se Met和Se Et的线性范围分别是1.51500μg Se/L和2.22200μg Se/L,各硒形态的线性相关系数均不少于0.9997。将该方法应用于食品中硒形态的分析,其加标回收的回收率为80.8%106.7%,相对标准偏差(RSDs,n=3)为4.7%9.6%。方法具有操作简单、方便快速、灵敏度高和环境友好等优点,可满足食品中硒形态定量分析。 
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  • 收稿日期:  2016-07-25

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