Abstract: Zhengdan 958 maize was chosen as the research object in this paper and it was stored under four different microenvironments,which were simulated to Meng- Xin,north China,central China and south China( 15 ℃,50% RH; 20 ℃,65% RH; 28 ℃,75% RH; 35 ℃,85% RH),to explore the variation law of maize protein during storage.Results showed that there was no significant influence of storage time on total protein content in each treatment group( p > 0.05); the contents of albumin,globulin,gliadin and sulfhydryl all presented descending tendencies,while the contents of gluten and disulfide bond showed rising tendencies( p < 0.05).The contents of protein components,sulfydryl and disulfide bond were changed significantly( p < 0.05) under different storage microenvironments.Compared with the conditions of high temperature and humidity( 35 ℃,85% RH) and low temperature and humidity( 15 ℃,50% RH),the contents of albumin,globulin,gliadin and sulfhydryl were reduced by 0.24,0.13,0.26 g /100 g and 1.19 μmol / g,while the contents of gluten and disulfide bond were increased by0.74 g /100 g and 2.05 μmol / g.In summary,temperature and humidity can be used as a reference index for the quality change of maize,and low temperature and humidity environment( 15 ℃,50% RH) was more conducive to the safe storage of maize.