Abstract: In order to develop a foundation for industrial production and utilization in the future. The basic composition and content of different parts of the bovine bone from Luxi,Qinchuan,Yanbian,Nanyang cattle and Lhasas yak had been analyzed in this study.The nutrient composition including moisture,ash,protein and fat,the amino acids,hydroxyproline( Hyp),mineral and fatty acids were identified.Results showed that the bovine bone consists of 13.70% ~ 32.94% moisture,35.75% ~ 48.07%ash,16.67% ~24.85% protein,8.15% ~ 26.78% fat and 5.81 ~ 8.24 mg / g bone collagen that 29.34% ~ 44.58% of the total protein content.The results showed that the bovine bones were rich in essential amino acids,calcium,iron,zinc,selenium and other major and trace elements that were essential to the human physiology,characteristic fatty acid of bovine bone were C18∶ 1n9 c,C16∶ 0and C18∶ 0.Different varieties and different parts of the bone were differences,Luxi cattle bones had the higher fatty acid contents while yak bones had a high content with protein and the amino acids.