Abstract: The perennial woody vines Ampelopsis grossedentata was used as materials for pot experiment,to selecte the suitable concentration of selenium fertilizer by exploring selenium changes and growth of Ampelopsis grossedentata under the conditions of different concentrations of Selenium. This experiment aimed at providing the basis for the large- scale cultivation and utilization of selenium- rich Ampelopsis grossedentata. The main results was as follows: a certain concentration of selenium fertilizer had significant influence on promoting the root,stem and leaf growth and inflorescence weight. With the increase of concentration of selenium treatment,the selenium content of Ampelopsis grossedentata showed elevation overall. When selenium concentration increased to a certain extent,selenium content of Ampelopsis grossedentata was not significantly increased.Comprehensive selenium enrichment and growth of effect showed that selenium concentrations of selenium enriched Ampelopsis grossedentata was 5~10 mg / kg.This range of concentration of selenium on the growth and development had a positive impact and Ampelopsis grossedentata high selenium content.