HPLC-ELSD法测定三种植物基原金线莲的金线莲苷含量
Content determination of kinsenoside in Jin-Xian-Lian from three Anoectochilus species by HPLC-ELSD
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摘要: 建立HPLC-ELSD法,测定三种植物基原金线莲中金线莲苷含量。采用phenomenex NH2色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水（92∶8,V/V）为流动相进行等度洗脱,流速为0.8 m L/min,柱温室温,ELSD雾化室温度为70℃,氮气流速为1.5 L/min。实验结果表明,金线莲苷进样量在76.022432.64μg/m L范围内线性良好（r=0.9991）;精密度、稳定性、重复性实验的RSD均≤3%;平均加样回收率为97.55%,RSD为1.50%（n=6）。33批次金线莲的金线莲苷含量在6.37%22.66%之间,平均含量为15.00%,存在较大的差异（RSD=26.29%）。该方法快速、简便,结果准确、可靠,适用于金线莲中金线莲苷的定量分析。Abstract: To establish a HPLC- ELSD method for determination of kinsenoside in Jin- Xian- Lian from three Anoectochilus species,the HPLC system consisting of phenomenex NH2column( 250 mm × 4.6 mm,5 μm) and an isocratic elution system of acetonitrile- water( 92∶ 8,V / V) as the mobile phase was adopted.The flow rate was set at 0.8 m L / min,the column temperature was room temperature,ELSD spray chamber temperature was 70 ℃,and the nitrogen flow rate was 1.5 L / min. The results indicated that the liner range of kinsenoside was 76.02 ~ 2432.64 μg / m L( r = 0.9991); RSD of precision,stability and reproducibility tests were no more than 3%; average recovery rate was 97.55%( RSD = 1.50%,n = 6). The contents of kinsenoside in Jin- Xian- Lian from three Anoectochilus species were in the range of 6.37% ~22.66%,and the average content was 15.00%( RSD = 26.29%). This method was quick,simple,accurate and reliable,and could be used to determine the content of kinsenoside in Jin- Xian- Lian.
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