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中国精品科技期刊2020
陈霞, 李兴正, 徐桂云, 郑江霞. 鸡蛋产品鉴伪技术的构建[J]. 食品工业科技, 2017, (01): 300-303. DOI: 10.13386/j.issn1002-0306.2017.01.052
引用本文: 陈霞, 李兴正, 徐桂云, 郑江霞. 鸡蛋产品鉴伪技术的构建[J]. 食品工业科技, 2017, (01): 300-303. DOI: 10.13386/j.issn1002-0306.2017.01.052
CHEN Xia, LI Xing-zheng, XU Gui-yun, ZHENG Jiang-xia. An authentication technology of egg products[J]. Science and Technology of Food Industry, 2017, (01): 300-303. DOI: 10.13386/j.issn1002-0306.2017.01.052
Citation: CHEN Xia, LI Xing-zheng, XU Gui-yun, ZHENG Jiang-xia. An authentication technology of egg products[J]. Science and Technology of Food Industry, 2017, (01): 300-303. DOI: 10.13386/j.issn1002-0306.2017.01.052

鸡蛋产品鉴伪技术的构建

An authentication technology of egg products

  • 摘要: 本实验建立了一种综合蛋白质和基因组两种检测方法的鸡蛋产品鉴伪技术。一方面,研究选择鸡蛋蛋清中的溶菌酶、卵白蛋白、伴白蛋白作为蛋清蛋白质的标志性成分,采用改进后的离子交换树脂法对上述三种目标蛋白进行分离,经聚丙烯酰胺凝胶电泳分析从而实现蛋白质水平的鉴定。另一方面,针对鸡蛋产品中常见的掺假物玉米粉、大豆粉,以高等植物18S r DNA基因作为内源参照基因,设计这两种掺假物的特异引物进行PCR扩增,经过琼脂糖凝胶电泳显示特异性条带的有无,从基因组水平实现鸡蛋产品的鉴伪。结果表明:离子交换法对轻加工鸡蛋产品中有良好的鉴定效果,对深加工鸡蛋产品中蛋白质的提取效果欠佳;PCR反应设计的引物特异性良好,鸡蛋产品中掺杂的最低检测限可高达2%。结合离子交换树脂和PCR法两种检测手段可对市售大部分鸡蛋产品进行鉴定。 

     

    Abstract: An authentication technology of egg products were built in this article,which integrated both protein and genomic testing methods. On one hand,lysozyme,ovalbumin and conalbumin were regarded as the iconic ingredients in egg white protein.Three proteins were separated by the optimized ion-exchange resin method,and were subjected to polyacrylamide gel electrophoresis to achieve the identification from protein level. On the other hand,because corn flour and soybean powder adulterants were found in egg products,a testing method from the genome level was proposed in this research. The 18 S rDNA genes in higher plant as the endogenous reference gene,species specific primers from adulterated substances were designed,and were amplified by Polymerase Chain Reaction( PCR). Through the presence or absence of specific bands on the Agarose Gel Electrophoresis gel,the egg product,can be easily identified from the genome level. The result showed that ion-exchange was effective method on authentication the a little bit egg food processing,but was not so good to extraction proteins from the overprocessing egg foods. The designed primers had strong specificities,the detection limitation was 2% from the PCR result.Combine with two technologies of ion-exchange and PCR can authentication most of egg food from market.

     

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