复合菌固态发酵猪骨素产谷氨酸培养基优化

Optimization of medium constituents for Glutamic acid production by mixed starter cultures in pig ossein solid state fermentation

摘要: 优化米曲霉-红曲霉复合菌固态发酵猪骨素产谷氨酸的固态培养基以提高谷氨酸产量。通过单因素实验和正交实验,以谷氨酸产量为指标,研究培养基中碳源、氮源和表面活性剂对谷氨酸产量的影响并对其进行优化。结果表明,复合菌固态发酵的最佳固态培养基组分为麸皮100 g,猪骨素600 g,硫酸铵15 g,三聚甘油单硬脂酸酯3 g,补水至1000 g。在此条件下,30℃发酵5 d,谷氨酸产量达到167.8 g/kg,总氨基酸产量达到728.6 g/kg。

Abstract: The medium constituents for producing Glutamic acid by a mixed starter culture composed of Aspergillus oryzae and Monascus purpureus in pig ossein solid state fermentation were optimized. The effects of carbon sources,nitrogen sources and surfactants on the yield of Glutamic acid and total amino acids were studied by single factor and orthogonal array design methods. Results suggested that the optimal medium constituents were bran concentration of 100 g,pig ossein concentration of 60 g,( NH4)2SO4concentration of 15 g and tripolycerol monostearates concentration of 3 g.Under these conditions,the yields of Glutamic acid and total amino acids were167.8 g / kg and 728.6 g / kg,respectively,after a fermentation 5 days at 30 ℃.