Abstract: In order to provide academic foundation for refreshing and processing of Portunus trituberculatus,E- Nose and HS- SPME- GC- MS were used to investigate the effects of frozen storage time on volatile components in the muscle and ovary of Portunus trituberculatus. The results of electronic nose had showed that there were many differences between the odor of fresh crab muscles and that of frozen crab muscles.However,it was similar among the early stage of the frozen( 1,3,5 d,and 7 d),as well as the late stage of the frozen( 9,11,13 d and 15 d).The changes of odor components in crab ovaries were similar with those in muscle. The results of GC- MS indicated that the contents of esters in fresh crab and ovary were higher. With the extension of frozen storage time,the contents of esters decreased,while the contents of alkanes were increased. Compared to the early stage of the frozen,the contents of amines in muscle and ovary were risen at the late stage of the frozen.The results showed that the odor of frozen and fresh in crab muscle and ovaries were different.The frozen storage time was an important factor which affects the components of flavor substances in crab.