银杏酚C15∶1与鲱鱼精DNA相互作用的光谱研究

Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

摘要: 目的:研究银杏酚C15∶1与DNA的相互作用情况。方法:吖啶橙（AO）为荧光探针,在293 K和310 K p H7.4的Tris-HCl缓冲液中,采用荧光光谱法、粘度法和热溶解实验研究银杏酚C15∶1和鲱鱼精DNA的相互作用方式。结果:银杏酚C15∶1与AO-DNA之间的猝灭方式为静态猝灭,根据热力学参数确定作用力类型是以氢键作用为主,判断银杏酚C15∶1与AO-DNA之间的作用方式主要是嵌插作用。结论:粘度法及热变性实验结果进一步证明银杏酚C15∶1与AO-DNA之间的主要作用方式是嵌插模式。

Abstract: Objective: To study the interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA. Methods: The interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA was investigated by fluorescence spectroscopy,viscosity measurements and thermal dissolution assay in the Tris- HCl buffer( p H7.4) at 293 K and 310 K,Acridine Orange( AO) as the fluorescent probe.Results: The fluorescence quenching of Ginkgol C15 ∶ 1 by AO- DNA was a static quenching procedure. The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicated the interaction between Ginkgol C15 ∶ 1 and AO- DNA was intercalation. Conclusions:The results of viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15∶ 1 and AO- DNA.