Abstract: In order to reduce the cost of bacitracin industrial fermentation,improve the bacitracin a production,single factor experiment was used to looking for cheap ingredients to replace soybean flour and corn flour. And screened industrial culture medium was optimized by the Box- Behnken response surface method.Results showed that,wheat bran could replace corn flour and bean flour,as carbon and nitrogen sources in fermentation medium,reduces the cost of fermentation medium cost. The screened medium was: bran 50.3 g / L,ammonium sulfate,1.53 g / L,potassium dihydrogen phosphate 1.43 g / L,the predicted maximum yield was 1.70 g / L under this condition.Through certification,Bacitracin A production could reach 1.78 g / L.This paper was found that,using the optimized medium,the ability of produce spore by bacterium was high,the number of spore could reach 1.25 ×1011CFU / m L. The culture medium could achieve high yield,cost down and high spore formation rate at the same time.