Abstract: A strain with high inulinase productivity named C- 56 which was isolated from Jerusalem artichoke rhizosphere soil was identified.Analysis of 16 S rRNA gene sequence indicated that strain C- 56 was belonged to genus Burkholderia.The optimal carbon sources,nitrogen sources,and inorganic salt were identified as inulin,yeast extract,Mg SO4·7H2O through single factor test,respectively. The optimization of inulinase production from strain C-56 was studied by Taguchi method. Statistical analysis of the experimental data indicated that inulin was the most significant factor for inulinase production. The optimal medium compositions for maximizing the inulinase production were established as 50 g / L inulin,20 g / L yeast extract,6 g / L Mg SO4·7H2O,initial p H6.0. Under optimum conditions,( 29.34 ± 1.95) U / m L of inulinase activity were obtained,which increased about 3.69 times compared with the initial enzyme activity( 6.25 ± 0.84) U / m L.