Abstract: DNA barcoding as a novel molecular biologic technology has been widely used in the identification of distinguishing genetic relationship among species.This technology can be fast and accurate to detect the quality of meat products. DNA barcode has become one of the frontier areas of the most rapidly developing biologic technology,and it is based on a wide range of species gene database information,so it has good prospects in the meat science study.The basic principles of DNA barcode technology was discussed in this review and compared with other technologies based on DNA level.The DNA barcode technology's application in species identification,meat quality,safety analysis and commercial fraud were involved in this paper. The application of DNA barcode technology also was prospected in the future meat science.