Abstract: Objective: To optimize extraction technology and to observe the nootropic effect of Shenmaixingnaofang.Methods: With the transfer rates of lobetyolin and codonopsis pilosula polysaccharide,as evaluation indexes,the effect of the three factors,such as ultrasonic time,extraction temperature and solid to liquid ratio was investigated by the single factor experiment. By analysis of variance,the key factors that could influence the process were determined.Then the range of parameters of key factors was further studied and explored by Box- Behnken response surface methodology. The changes of learning and memorizing ability were tested with Morris water maze.Results: The optimum preparation conditions of the lobetyolin and codonopsis pilosula polysaccharide were as follows: ultrasonic time was 32 min,extraction temperature was 52 ℃,solid to liquid ratio was 1∶ 27.Under these conditions,the transfer rates of lobetyolin and codonopsis pilosula polysaccharide were( 5.18 ± 0.22) μg / g and2.07% ± 0.15%. Shenmaixingnaofang could improve the damage of learning and memory by D- gal and Na NO2.Conclusion: The optimum preparation conditions of the lobetyolin and codonopsis pilosula polysaccharide had been optimized by the response surface method,which could help to enhance the stability of the process. All the treatment groups diagnosed with Shenmaixingnaofang could improve the abilities of learning and memory in mice models.