响应面法优化玉米黄粉蛋白水解工艺

Optimization of hydrolysis technology of corn gluten meal by response surface methodology

摘要: 采用碱性蛋白酶制备高水解度的玉米蛋白酶解物,以水解度为考察指标,设计单因素及响应面实验,优化其水解工艺条件。实验结果表明:最佳水解工艺条件为底物浓度23 g/L,加酶量(E/S)3.5%,酶解温度49.7℃,酶解时间4 h,p H为9.0,在此条件下,水解度可达到27.18%。

Abstract: The corn polypeptide with high degree of hydrolysis( DH) was prepared by alkaline protease,with corn protein as raw material and degree of hydrolysis as detecting index by mono- factor analysis and response surface methodology.The results showed that under the optimal conditions: substrate concentration 23 g / L,proteases dosage 3.5%,hydrolysis temperature 49.7 ℃,hydrolysis time 4 h and p H 9.0,the degree of hydrolysis was 27.18%.