Abstract: Technological parameters of semi- bionic extraction( SBE) flavones from chestnut flower were optimized using single factor and orthogonal experiment,and the antibacterial activity of the flavones was investigated by measuring the inhibition zone and the minimum inhibitory concentration( MIC).The results showed that the optimum extracting conditions were extracting time 10 min,the temperature 80 ℃ and material- to- liquid ratio 1∶ 20( m∶ V).Under these optimal conditions,the extraction yield of( 6.76% ± 0.11%) were obtained. The flavones from chestnut flower exhibited the antibacterial activity against E.coli,Bacillus subtilis and Saccharomyces cerevisiae. The orders from strong to weak were Bacillus subtilis,E. coli and Saccharomyces cerevisiae. The minimum inhibition concentration of the flavonoids was respectively 2 mg / m L,5 mg / m L and 6 mg / m L.