Abstract: Selenide rice proteins( Se- RP) were prepared via rice proteins obtained by enzymatic hydrolysis were chemically modified,then their antioxidant activities were identified. The results showed that Se- RPs possessed certain antioxidant activity of glutathione peroxidase( GPX),wherein GPX activity of Se- RP24 was highest,approximately 116 U / g. Andvarious kinds of Se- RPs could play a scavenging role in free radical such as 1,1- diphenyl-2- three nitrophenylhydrazine( DPPH ·),hydroxyl radical( OH ·) and superoxide anion( O).Furthermore,Se- RP could effectively maintain the normal function of cardiac mitochondria,inhibit swelling of damaged mitochondria induced by ascorbic acid / divalent iron ions( V_C/ Fe~(2+)),and decrease content of the lipid oxidation product MDA in mitochondria.Se- RP sobtained successfully laid the experimental foundations for large scale preparation of plant- derived selenide protein,and also provided new insight for the development of new albuminoidal antioxidant drugs.