Abstract: The sugars and organic acids of ten blueberry cultivars from the North American were analyzed by HPLC( high performance liquid chromatography) method.The results indicated that the soluble sugars in blueberry were fructose and glucose,fructose content was from 18.916 ~ 73.899 mg / g,glucose content was from 19.194 ~79.521 mg / g. The organic acid components in blueberry included oxalic acid,quinic acid,malic acid,vitamin C and citric acid.The content of citric acid was from 1.174 to 15.069 mg / g,much more than others.Oxalic acid content was lowest,from 0.018 to 0.046 mg / g. There were some obvious differences in composition and content of sugar and organic acid among ten blueberry cultivars. Beikong exhibited the highest levels of fructose and glucose in comparison to the others.The berries of Elizabeth contained highest citric acid among all the cultivars.The value of sugars / total acids and sweetness / total acids were important indicator to measure the sweet and sour flavors of blueberry.The sugars / total acids were from 2.342 to 27.488 among ten blueberry cultivars. The sugars / total acids was from 285.953 to 3393.854,and Northsky > Chignecto > St.Cloud > Frendship > Blomidon > Northland > Blueray >Patriot > Brigitta > Elizabeth.