Abstract: Objective:To observe the effect of total flavonoids of mulberry leaves on liver in type 2 diabetic rats.Methods:The male wistar rats were fed with high fat diet and streptozotocin to induce the type 2 diabetic model and the model rats were divided into five groups randomly:model group,metformin group,total flavonoids of mulberry leaves dose groups(200,100,50 mg·kg^-1·bw^-1i.g). After 4 weeks,levels of weight,blood glucose,organ coefficient,total cholesterol(TC),triglyceride(TG) and alanine aminotransferase(ALT) in serum were observed;hepatic glycogen,hepatic GK activity,and liver samples were observed pathologically with HE staining. Results:Compared with the model group,total flavonoids of mulberry leaves high dose group could reduce the levels of weight(390.79±77.27) g,TC(2.25±0.58) mmol·L^-1,TG(0.92±0.36) mmol·L^-1 and ALT(69.36±5.58) U·L^-1,increase the content of hepatic glycogen(12.22±1.50) mg·g^-1,improve the activity of GK( 10. 33 ± 1. 30) m IU·min^-1·mg protein^-1, obviously alleviate the histopathologic changes including fatty degeneration. Conclusion:It showed that total flavonoids of mulberry leaves had potential to be developed in the aspect of preventing and improving liver damage in type 2 diabetic.