两株嗜酸乳杆菌体外降解粘蛋白能力的研究

Study on mucin degradation activity of two strains of Lactobacillus acidophilus in vitro

摘要: 以从人体肠道中筛选,并经16S r DNA鉴定的2株嗜酸乳杆菌KLDS1.0901、KLDS1.0902为研究对象,体外评估两株嗜酸乳杆菌降解粘蛋白的能力。体外粘蛋白降解能力的评估主要是通过液体培养法、SDS-PAGE蛋白分析以及平板降解实验三部分进行。受试菌株在含粘蛋白的液体培养基中生长不良,粪便菌群生长良好;SDS-PAGE粘蛋白残留分析检测以及平板粘蛋白溶圈检测表明受试菌株无粘蛋白降解活性,而粪便菌群产生阳性反应。实验结果表明,两株嗜酸乳杆菌无体外降解粘蛋白活性。

Abstract: In this study,the ability to degrade mucin of two strains of Lactobacillus acidophilus(KLDS1.0901 and KLDS1.0902) screened from the human intestine and identified by 16 S r DNA in vitro was evaluated. Mucinolytic activity was examined using the following three tests:growth in liquid medium,SDS-PAGE analysis of degraded mucin residues,and degradation assay in Petri dish. The test strains fell to grow in the liquid medium containing mucin,while fecal sample grew well. In the SDS-PAGE analyses of mucin residues and observation of mucinolytic zone in agar plate,the two test strains also showed no mucin degradation activity,while fecal sample yielded positive results. The results demonstrated that strains tested were unable to degrade gastrointestinal mucin in vitro.