高产γ-氨基丁酸乳酸菌菌株的诱变选育与鉴定

Study on mutation breeding and identification of lactic acid bacteria for γ- aminobutyric acid production

摘要: 本文以从内蒙古传统发酵食品中分离的80株乳酸菌为研究对象,在GYP培养基中进行高产γ-氨基丁酸（GABA）菌株的筛选后,利用紫外线进行诱变处理,得到GABA突变菌株,并对其进行了菌种鉴定。结果表明,从80株供试乳酸菌中筛选出4株高产γ-氨基丁酸的菌株,再经紫外诱变后得到1株高产突变菌株US3-3。该菌株紫外诱变后,其γ-氨基丁酸含量为2.482 g/L,是诱变前提高1.9倍,并对其多次传代稳定性较好,经16S r DNA序列分析,鉴定为乳酸片球菌（Pediococcus acidilactici）。

Abstract: The growth ability of 80 lactic acid bacteria strains isolated from the traditional fermented food in Inner Mongolia were screened according to the γ- aminobuyric acid( GABA) accumulation amount in the GYP medium.The high- yield GABA strains were obtained by ultraviolet mutagenesis,and then the strain was identified by16 S r DNA gene sequences.The results showed that 4 high- yield GABA strains were screened out from 80 strains of lactic acid bacteria and a high yield GABA producing strain US3- 3 was obtained by UV mutagenesis and its content of GABA was 2.482 g / L,which was 1.9 times as much as original strain.The strain US3-3 was identified as Pediococcus acidilactici.