Abstract: In this work,bovine serum albumin molecules and hydrophobic flavonols was used to formed bovine serum albumin / flavonols nano core- shell particles,the size of nanoparticle was determined by TEM,the adsorption capacity was analyzed by HPLC and the antioxidant activity of flavonol before and after being embedded was evaluated by DPPH and ABTS radical scavenging assays.The result demonstrated that the size of nanoparticle was 20 nm.The conjugation of bovine serum albumin and flavonols affected the encapsulation of BSA.Moreover,the antioxidant activity group of flavonols formed an intermolecular hydrogen bond with BSA,making the activities of flavonols were hindered by the embedding of bovine serum albumin nanoparticle. The preparation of BSA / flavonoids nano core- shell particles would be a new way to promote the stability of flavonoids,preserve the antioxidant activity and achieve a higher bioavailability of flavonoids.