Abstract: In order to find a new type of water-holding agent of aquatic products to solve the problem of excess use of phosphate,the grass carp viscera was alkaline hydrolyzed to obtain the hydrolysed protein. The hydrolysed protein was used to hold the water of Lateolabrax japonicus fillets in this paper. The soaking increase yield,thawing loss yield,cooking loss yield of the Lateolabrax japonicus fillets were used to optimize water-holding capacity of hydrolysed protein. The water status,texture profiles,and color changes of Lateolabrax japonicus fillets were analyzed by low field nuclear magnetic resonance(NMR) instrument,texture instrument and colorimeter to analyze the effect on Lateolabrax japonicus fillets in water-holding capacity. The results showed that the optimun hydrolysed p H value was 11 and the condition of holding water was 4% protein liquid mixed with 0.15% composite phosphate at 10 ℃ for 2 h. The thawing loss yield 28.33% less than composite phosphate treatment and the cooking loss yield 5.98% less than it. But the water- holding capacity was 4.94%more than composite phosphate treatment. The color of Lateolabrax japonicus fillets was improved more than composite phosphate treatment.