Abstract: In order to improve the conjugate conversion rate of linoleic acid in okra seed oil,the Box-Benhnken experimental design was performed on the basis of one-factor experiments,and the response surface analysis and corresponding regression model were established with conjugated linoleic acid(CLA) yield as response value. The optimum conditions of conjugate conversion were obtained as follows:reaction time of 4 h,reaction temperature of 162 ℃,oil against KOH at ratio of 1.9(m L/g),propylene glycol against oil at ratio of 2.0(m L/m L),and the conversion rate of CLA was 74.20%. Conversion rate was greatly affected by reaction temperature and the interaction of reaction time and temperature was significant. The measured date( 74.20%) was almost accorded with the predicted data(76.99%). It demonstrates that the conversion condition parameter optimized by response surface method was credible and feasible.