呋喃它酮代谢物直接竞争化学发光酶免疫分析法的建立

Development of a direct competitive chemiluminescent enzyme immunoassay for detection of furaltadone metabolite

摘要: 采用以对碘苯酚为增强剂的鲁米诺-辣根过氧化物酶-过氧化氢化学发光检测体系,建立检测动物组织中呋喃它酮代谢物5-吗啉甲基-2-氨基-2-僫唑烷基酮（AMOZ）残留的直接竞争化学发光酶免疫分析法（dc-CLEIA）。结果表明:此方法IC50为0.62 ng/m L,检测限为15.52 pg/m L,线性范围0.038⁹.78 ng/m L,变异系数均小于10%;该方法中抗体除了与呋喃它酮原药有一定交叉外,与其他结构类似物无交叉,表现了良好的特异性;鸡肉样品添加回收率为83%⁹4%,验证了该方法的准确性,为实际动物组织中AMOZ残留提供了便捷、准确、快速筛查的手段。

Abstract: A direct competitive chemiluminescent enzyme immunoassay method( dc-CLEIA) for detection of AMOZ which based on lodine phenol enhancer Luminol-HRP-H2O2 system was developed. The 3-amino-5-morpolinomethyl-2-oxazolidinone was metabolite of furaltdone. The sensitivity(IC50) and detection limit(LOD)of the method were 0.62 ng/m L and 15.52 pg/m L respectively. The linear range was 0.038 ~9.78 ng/m L. The relative standard deviations was below 10%. The antibody was high specific for furaltdone derivative and no cross-reactivity except the furaltdone original drug. The accuracy of the method was verified and the recovery in chicken tissues was 83%~94%. The method provided a convenient,accurate and rapid screening means for detecting AMOZ in actual animal tissue.